The Effects of Natural Epigenetic Therapies in 3D Ovarian Cancer and Patient-Derived Tumor Explants: New Avenues in Regulating the Cancer Secretome

High mortality rates in ovarian cancer have been linked to recurrence, metastasis, and chemoresistant disease, which are known involve not only genetic changes but also epigenetic aberrations. In cancer, adipose-derived stem cells from the omentum (O-ASCs) play a crucial role supporting tumor its tumorigenic microenvironment, further propagating abnormalities dissemination of disease. Epigallocatechin gallate (EGCG), DNA methyltransferase inhibitor derived green tea, Indole-3-carbinol (I3C), histone deacetylase cruciferous vegetables, carry promising effects reprograming aberrant modifications cancer. Therefore, we demonstrate action these diet-derived compounds suppressing growth 3D spheroids or organoids as well post-treatment recovery through proliferation, migration, invasion, colony formation assays when compared synthetic compound Panobinostat with without standard chemotherapy. Finally, given regulatory secretome growth, chemoresistance, relapse that natural can regulate secretion protumorigenic factors, cytokines, extracellular matrix components, immunoregulatory markers human specimens. While studies needed, our results suggest treatments could be considered future adjuncts chemotherapy, improving efficiency patient outcomes.